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Recombinant Human Melanoma Inhibitory Activity

Cat. No.: MIA-140H
Product Overview: Recombinant HumanMelanoma Inhibitory Activity produced inE.Coliis a single, non-glycosylated, polypeptide chain consisting of 108 amino having a total molecular mass of 12237 Dalton. The MIA is purified by proprietary chromatographic techniques.
Description: The Melanoma Inhibitory protein (MIA) was identified as an inhibitor of in vitro growth of malignant melanoma cells. The protein contains a SH3 domain. MIA acts as a potent tumor cell growth inhibitor for malignant melanoma cells and some other neuroectodermal tumors, including gliomas, in an autocrine fashion. In a study of human melanoma cell lines with different metastatic capacity MIA mRNA expression appeared to be inversely correlated with pigmentation. MIA has been shown to represent a very sensitive and specific serum marker for systemic malignant melanoma that might be useful for staging of primary melanomas, detection of progression from localized to metastatic disease during follow-up, and monitoring therapy of advanced melanomas.
Source: Escherichia Coli.
Amino Acid Sequence: Agrees with the sequence of native MIA human with an addition N-terminal Methionine residue.MGPMPKLADRKLCADQECSSHPISMAVALQDYMAPDCRFLTIHRGQVVYVFSLKGRGRFLWGGSVQGDYYGDLAARLGYFPSSIVREDQTLKVDVKTDKWDFYCQ.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation: The protein was lyophilized from a concentrated (1.66 mg/ml) solution containing 20 mM Potassium-phosphate pH=7 and 150 mM potassium chloride.
Specific Activity: The biological activity is calculated by the inhibiting effect on the invasion of Mel In Tumor cells (Bosserhoff et al.2003 Lab Invest. 83, 1583-94) and found active in Mel In assay.
Protein Content: UV spectroscopy at 280 nm using the absorption coefficient of 19300 M-1cm-1.
Solubility: It is recommended to reconstitute the lyophilized Melanoma Inhibitory Activity in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Gene Name: MIA melanoma inhibitory activity [ Homo sapiens ]
Synonyms: Melanoma-derived growth regulatory protein precursor, Cartilage-derived retinoic acid-sensitive protein, CD-RAP, MIA, melanoma inhibitory activity; Melanoma-derived growth regulatory protein
Gene ID: 8190
mRNA Refseq: NM_006533
Protein Refseq: NP_006524
MIM: 601340
UniProt ID: Q16674
Chromosome Location: 19q13.32-q13.33
Function: growth factor activity

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