Recombinant Human WNT1, StrepII-tagged

• Cat.No.: WNT1-204H
• Product Overview: Purified, full-length human recombinant WNT1 protein (amino acids 28-370, 343 a.a.) with StrepII tag, produced in human cells. Predicted molecular weight: 38.3 kDa. (Accession NP_005421.1; UniProt P04628)

Specification

• Description: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This protein is a member of the WNT family. It is very conserved in evolution, and the protein is 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum.
• Source: Human Cells
• Species: Human
• Tag: StrepII
• Form: Lyophilized from sterile 0.2μm filtered solution in 0.3X PBS with 10% Trehalose (carrier-free).
• Protein length: 28-370, 343 a.a.
• AA Sequence: ANSSGRWWGIVNVASSTNLLTDSKS LQLVLEPSLQLLSRKQRRLIRQNPG ILHSVSGGLQSAVRECKWQFRNRRW NCPTAPGPHLFGKIVNRGCRETAFI FAITSAGVTHSVARSCSEGSIESCT CDYRRRGPGGPDWHWGGCSDNIDFG RLFGREFVDSGEKGRDLRFLMNLHN NEAGRTTVFSEMRQECKCHGMSGSC TVRTCWMRLPTLRAVGDVLRDRFDG ASRVLYGNRGSNRASRAELLRLEPE DPAHKPPSPHDLVYFEKSPNFCTYS GRLGTAGTAGRACNSSSPALDGCEL LCCGRGHRTRTQRVTERCNCTFHWC CHVSCRNCTHTRVLHECL
• Endotoxin: <0.1 eu per ug protein by lal
• Purity: >95% pure by SDS-PAGE
• Storage: 12 months at -20°C as supplied; 1 month at 4??C after reconstitution. Avoid freeze/thaw cycles. - See more at: http://www.genemed.com/90001#sthash.FsM1acyN.dpuf
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in PBS to a concentration of 0.1 mg/ml.

Gene Information

• Gene Name: WNT1 wingless-type MMTV integration site family, member 1 [ Homo sapiens ]
• Official Symbol: WNT1
• Synonyms: WNT1; wingless-type MMTV integration site family, member 1; INT1; proto-oncogene Wnt-1; proto-oncogene Int-1 homolog; wingless-type MMTV integration site family, member 1 (oncogene INT1)
• Gene ID: 7471
• mRNA Refseq: NM_005430
• Protein Refseq: NP_005421
• MIM: 164820
• UniProt ID: P04628
• Chromosome Location: 12q13
• Pathway: Adipogenesis, organism-specific biosystem; Basal cell carcinoma, organism-specific biosystem; Basal cell carcinoma, conserved biosystem; C-MYB transcription factor network, organism-specific biosystem; Class B/2 (Secretin family receptors), organism-specific biosystem; DNA damage response (only ATM dependent), organism-specific biosystem; Developmental Biology, organism-specific biosystem
• Function: cytokine activity; frizzled binding; frizzled-2 binding; protein binding; protein domain specific binding; receptor agonist activity; transcription regulatory region DNA binding