Recombinant Human glutamyl-tRNA(Gln) amidotransferase, subunit C, His-tagged
Cat.No. : | GATC-229H |
Product Overview : | GATC, 1-136aa, Human, His tag, E.coli |
- Specification
- Gene Information
- Related Products
Description : | GATC allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). This protein is subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112) and C (GATC) subunits. Recombinant human GATC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Source : | E.coli |
Species : | Human |
Tag : | His-tag |
Form : | Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT |
Molecular Mass : | 17.5 kDa (159aa), confirmed by MALDI-TOF(Molecular size on SDS-PAGE will appear higher) |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MGSMWSRLVW LGLRAPLGGR QGFTSKADPQ GSGRITAAVI EHLERLALVD FGSREAVARL EKAIAFADRL RAVDTDGVEP MESVLEDRCL YLRSDNVVEG NCADELLQNS HRVVEEYFVA PPGNISLPKL DEQEPFPHS |
Purity : | >85% by SDS - PAGE |
Storage : | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Concentration : | 0.25 mg/ml (determined by Bradford assay) |
Gene Name : | GATC?glutamyl-tRNA(Gln) amidotransferase, subunit C [?Homo sapiens(human) ] |
Official Symbol : | GATC |
Synonyms : | GATC; 15E1.2; glutamyl-tRNA(Gln) amidotransferase, subunit C; glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial; glu-AdT subunit C; glutamyl-tRNA(Gln) amidotransferase, subunit C homolog; NP_789788.1; EC 6.3.5.- |
Gene ID : | 283459 |
mRNA Refseq : | NM_176818 |
Protein Refseq : | NP_789788 |
UniProt ID : | O43716 |
Chromosome Location : | 12q24.31 |
Pathway : | Aminoacyl-tRNA biosynthesis; mRNA processing |
Function : | ATP binding; glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity |
Products Types
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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