Recombinant Human ribosomal protein S19, His-tagged

• Cat.No.: RPS19-239H
• Product Overview: RPS19, 1-145aa, Human, His tag, E.coli

Specification

• Description: RPS19 belongs to the S19E family of ribosomal proteins. It is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia (DBA), a constitutional erythroblastopenia characterized by absent or decreased erythroid precursors, in a subset of patients. This suggests a possible extra-ribosomal function for this gene in erythropoietic differentiation and proliferation, in addition to its ribosomal function. Higher expression levels of this gene in some primary colon carcinomas compared to matched normal colon tissues has been observed. Recombinant human RPS19 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT
• Molecular Mass: 18.4kDa (168aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPGVTVK DVNQQEFVRA LAAFLKKSGK LKVPEWVDTV KLAKHKELAP YDENWFYTRA ASTARHLYLR GGAGVGSMTK IYGGRQRNGV MPSHFSRGSK SVARRVLQAL EGLKMVEKDQ DGGRKLTPQG QRDLDRIAGQ VAAANKKH
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: RPS19 ribosomal protein S19 [ Homo sapiens ]
• Official Symbol: RPS19
• Synonyms: RPS19; ribosomal protein S19; 40S ribosomal protein S19; DBA; Diamond Blackfan anemia; S19; DBA1
• Gene ID: 6223
• mRNA Refseq: NM_001022
• Protein Refseq: NP_001013
• MIM: 603474
• UniProt ID: P39019
• Chromosome Location: 19q13.2
• Pathway: Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S, organism-specific biosystem; Cap-dependent Translation Initiation, organism-specific biosystem; Cytoplasmic Ribosomal Proteins, organism-specific biosystem; Disease, organism-specific biosystem; Eukaryotic Translation Elongation, organism-specific biosystem; Eukaryotic Translation Initiation, organism-specific biosystem; Eukaryotic Translation Termination, organism-specific biosystem
• Function: RNA binding; protein binding; structural constituent of ribosome