Recombinant Human major histocompatibility complex, class I, C, His-tagged

• Cat.No.: HLA-C-268H
• Product Overview: HLA-C, 25-308aa, Human, His tag, E.coli

Specification

• Description: HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. Recombinant human HLA-C protien, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Molecular Mass: 34.9 kDa (307aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSCSHSMRY FDTAVSRPGR GEPRFISVGY VDDTQFVRFD SDAASPRGEP RAPWVEQEGP EYWDRETQKY KRQAQADRVS LRNLRGYYNQ SEDGSHTLQR MSGCDLGPDG RLLRGYDQSA YDGKDYIALN EDLRSWTAAD TAAQITQRKL EAARAAEQLR AYLEGTCVEW LRRYLENGKE TLQRAEPPKT HVTHHPLSDH EATLRCWALG FYPAEITLTW QRDGEDQTQD TELVETRPAG DGTFQKWAAV VVPSGQEQRY TCHMQHEGLQ EPLTLSWEPS SQPTIPI
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: HLA-C major histocompatibility complex, class I, C [ Homo sapiens ]
• Official Symbol: HLA-C
• Synonyms: major histocompatibility complex, class I, C; 4933; Ensembl:ENSG00000204525; FLJ27082; HLA class I histocompatibility antigen, Cw-1 alpha chain;MHC class I antigen heavy chain HLA-C;human leukocyte antigen-C alpha chain;major histocompatibility antigen HLA-C;HLA class I histocompatibility antigen, C alpha chain; HLC-C; D6S204; PSORS1; HLA-JY3
• Gene ID: 3107
• mRNA Refseq: NM_001243042.1
• Protein Refseq: NP_001229971.1
• MIM: 142840
• UniProt ID: O19617
• Chromosome Location: 6p21.3
• Pathway: Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Antigen Presentation: Folding, assembly and peptide loading of class I MHC, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Antigen processing-Cross presentation, organism-specific biosystem
• Function: MHC class I receptor activity