Recombinant Human ubiquitin-conjugating enzyme E2R 2, His-tagged

• Cat.No.: UBE2R2-291H
• Product Overview: UBE2R2, 1-238aa, Human, His tag, E.coli

Specification

• Description: UBE2R2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This protein is a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation. Recombinant human UBE2R2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Molecular Mass: 29.6kDa (261aa) confirmed by MALDI-TOF(Molecular size on SDS-PAGE will appear higher)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAQQQMT SSQKALMLEL KSLQEEPVEG FRITLVDESD LYNWEVAIFG PPNTLYEGGY FKAHIKFPID YPYSPPTFRF LTKMWHPNIY ENGDVCISIL HPPVDDPQSG ELPSERWNPT QNVRTILLSV ISLLNEPNTF SPANVDASVM FRKWRDSKGK DKEYAEIIRK QVSATKAEAE KDGVKVPTTL AEYCIKTKVP SNDNSSDLLY DDLYDDDIDD EDEEEEDADC YDDDDSGNEE S
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: UBE2R2 ubiquitin-conjugating enzyme E2R 2 [ Homo sapiens ]
• Official Symbol: UBE2R2
• Synonyms: UBE2R2; ubiquitin-conjugating enzyme E2R 2; ubiquitin-conjugating enzyme E2 R2; CDC34B; FLJ20419; MGC10481; UBC3B; ubiquitin-protein ligase R2; ubiquitin carrier protein R2; ubiquitin-conjugating enzyme UBC3B; ubiquitin-conjugating enzyme E2-CDC34B; E2-CDC34B
• Gene ID: 54926
• mRNA Refseq: NM_017811
• Protein Refseq: NP_060281
• MIM: 612506
• UniProt ID: Q712K3
• Chromosome Location: 9p11.2
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem
• Function: ATP binding; acid-amino acid ligase activity; ligase activity; nucleotide binding; ubiquitin-protein ligase activity