Recombinant Human protein kinase, AMP-activated, beta 1 non-catalytic subunit, His-tagged

• Cat.No.: PRKAB1-347H
• Product Overview: PRKAB1, 1-270aa, Human, His tag, E.coli

Specification

• Description: 5"-AMP-activated protein kinase subunit beta-1, also known as PRKAB1, inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). Recombinant human PRKAB1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 32.8 kDa (293aa) confirmed by MALDI-TOF(Molecular size on SDS-PAGE will appear higher)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGNTSSE RAALERHGGH KTPRRDSSGG TKDGDRPKIL MDSPEDADLF HSEEIKAPEK EEFLAWQHDL EVNDKAPAQA RPTVFRWTGG GKEVYLSGSF NNWSKLPLTR SHNNFVAILD LPEGEHQYKF FVDGQWTHDP SEPIVTSQLG TVNNIIQVKK TDFEVFDALM VDSQKCSDVS ELSSSPPGPY HQEPYVCKPE ERFRAPPILP PHLLQVILNK DTGISCDPAL LPEPNHVMLN HLYALSIKDG VMVLSATHRY KKKYVTTLLY KPI
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: PRKAB1 protein kinase, AMP-activated, beta 1 non-catalytic subunit [ Homo sapiens ]
• Official Symbol: PRKAB1
• Synonyms: PRKAB1; protein kinase, AMP-activated, beta 1 non-catalytic subunit; 5-AMP-activated protein kinase subunit beta-1; AMPK beta 1; AMPKb; AMPK beta -1 chain; AMPK subunit beta-1; AMP-activated protein kinase beta subunit; 5-AMP-activated protein kinase beta-1 subunit; protein kinase, AMP-activated, noncatalytic, beta-1; AMPK; HAMPKb; MGC17785
• Gene ID: 5564
• mRNA Refseq: NM_006253
• Protein Refseq: NP_006244
• MIM: 602740
• UniProt ID: Q9Y478
• Chromosome Location: 12q24.1-q24.3
• Pathway: AMPK signaling, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Direct p53 effectors, organism-specific biosystem; Energy Metabolism, organism-specific biosystem; Energy dependent regulation of mTOR by LKB1-AMPK, organism-specific biosystem; Hypertrophic cardiomyopathy (HCM), organism-specific biosystem
• Function: AMP-activated protein kinase activity; protein binding; protein kinase binding