Recombinant Human troponin I type 1 (skeletal, slow), His-tagged

• Cat.No.: TNNI1-352H
• Product Overview: TNNI1, 1-187aa, Human, His tag, E.coli

Specification

• Description: Troponin I, slow skeletal muscle, also known as TNNI1, belongs to the troponin I family. TNNI1 is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Recombinant human TNNI1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Molecular Mass: 23.8 kDa (207aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MPEVERKPKI TASRKLLLKS LMLAKAKECW EQEHEEREAE KVRYLAERIP TLQTRGLSLS ALQDLCRELH AKVEVVDEER YDIEAKCLHN TREIKDLKLK VMDLRGKFKR PPLRRVRVSA DAMLRALLGS KHKVSMDLRA NLKSVKKEDT EKERPVEVGD WRKNVEAMSG MEGRKKMFDA AKSPTSQ
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TNNI1 troponin I type 1 (skeletal, slow) [ Homo sapiens ]
• Official Symbol: TNNI1
• Synonyms: TNNI1; troponin I type 1 (skeletal, slow); troponin I, skeletal, slow; troponin I, slow skeletal muscle; troponin I, slow-twitch isoform; TNN1; SSTNI; DKFZp451O223
• Gene ID: 7135
• mRNA Refseq: NM_003281
• Protein Refseq: NP_003272
• MIM: 191042
• UniProt ID: P19237
• Chromosome Location: 1q31.3
• Pathway: Muscle contraction, organism-specific biosystem; Striated Muscle Contraction, organism-specific biosystem; Striated Muscle Contraction, organism-specific biosystem
• Function: actin binding; tropomyosin binding