Recombinant Human Ras association (RalGDS/AF-6) domain family member 1, His-tagged

• Cat.No.: RASSF1-376H
• Product Overview: RASSF1A, 1-340aa, Human, His tag, E.coli

Specification

• Description: RASSF1A is similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. This protein required for death receptor-dependent apoptosis. Recombinant human RASSF1A protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
• Molecular Mass: 42.9 kDa (376aa)
• AA Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSGE PELIELRELA PAGRAGKGRT RLERANALRI ARGTACNPTR QLVPGRGHRF QPAGPATHTW CDLCGDFIWG VVRKGLQCAH CKFTCHYRCR ALVCLDCCGP RDLGWEPAVE RDTNVDEPVE WETPDLSQAE IEQKIKEYNA QINSNLFMSL NKDGSYTGFI KVQLKLVRPV SVPSSKKPPS LQDARRGPGR GTSVRRRTSF YLPKDAVKHL HVLSRTRARE VIEALLRKFL VVDDPRKFAL FERAERHGQV YLRKLLDDEQ PLRLRLLAGP SDKALSFVLK ENDSGEVNWD AFSMPELHNF LRILQREEEE HLRQILQKYS YCRQKIQEAL HACPLG
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: RASSF1 Ras association (RalGDS/AF-6) domain family member 1 [ Homo sapiens ]
• Official Symbol: RASSF1
• Synonyms: RASSF1; Ras association (RalGDS/AF-6) domain family member 1; ras association domain-containing protein 1; 123F2; NORE2A; RDA32; REH3P21; WUGSC:H_LUCA12.5; tumor suppressor protein RDA32; cardiac-specific ras association domain family 1 protein; pancreas-specific ras association domain family 1 protein; RASSF1A
• Gene ID: 11186
• mRNA Refseq: NM_001206957
• Protein Refseq: NP_001193886
• MIM: 605082
• UniProt ID: Q9NS23
• Chromosome Location: 3p21.3
• Pathway: Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Non-small cell lung cancer, organism-specific biosystem; Non-small cell lung cancer, conserved biosystem; Pathways in cancer, organism-specific biosystem; p53 pathway, organism-specific biosystem
• Function: identical protein binding; metal ion binding; protein N-terminus binding; protein binding; zinc ion binding