Recombinant Human polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa, His-tagged

• Cat.No.: POLR2I-389H
• Product Overview: POLR2I, 1-125aa, Human, His tag, E.coli

Specification

• Description: POLR2I, also known as RPB9, belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family. This protein is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Also it is synthesizes mRNA precursors and many functional non-coding RNAs. Recombinant human PORL2I protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 1mM DTT
• Molecular Mass: 17.0kDa (148aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEPDGTY EPGFVGIRFC QECNNMLYPK EDKENRILLY ACRNCDYQQE ADNSCIYVNK ITHEVDELTQ IIADVSQDPT LPRTEDHPCQ KCGHKEAVFF QSHSARAEDA MRLYYVCTAP HCGHRWTE
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: POLR2I polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa [ Homo sapiens ]
• Official Symbol: POLR2I
• Synonyms: POLR2I; polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa; polymerase (RNA) II (DNA directed) polypeptide I (14.5kD); DNA-directed RNA polymerase II subunit RPB9; hRPB14.5; RPB9; RPB14.5; RNA polymerase II subunit B9; RNA polymerase II 14.5 kDa subunit; DNA-directed RNA polymerase II subunit I; DNA-directed RNA polymerase II 14.5 kDa polypeptide
• Gene ID: 5438
• mRNA Refseq: NM_006233
• Protein Refseq: NP_006224
• MIM: 180662
• UniProt ID: P36954
• Chromosome Location: 19q12
• Pathway: Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; DNA Repair, organism-specific biosystem; Disease, organism-specific biosystem; Dual incision reaction in TC-NER, organism-specific biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem; Formation of HIV-1 elongation complex containing HIV-1 Tat, organism-specific biosystem; Formation of HIV-1 elongation complex in the absence of HIV-1 Tat, organism-specific biosystem
• Function: DNA binding; DNA-directed RNA polymerase activity; metal ion binding; contributes_to protein kinase activity; zinc ion binding