Recombinant Human D-tyrosyl-tRNA deacylase 1, His-tagged

• Cat.No.: DTD1-392H
• Product Overview: DTD1, 1-209aa, Human, His tag, E.coli

Specification

• Description: DTD1 hydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr). It could be a defense mechanism against a harmful effect of D-tyrosine. DTD1 may be a risk factor for aspirin-intolerant asthma (AIA) by catalyzing the hydrolysis of D-tryptophan and interacting with the tyrosyl-tRNA synthetase (tyrRS) enzyme, which promotes a pro-inflammatory phenotype. This protein is localize to the DUE(DNA unwinding element )s of active replication origins. Recombinant human DTD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 25.9kDa (232aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMKAVVQR VTRASVTVGG EQISAIGRGI CVLLGISLED TQKELEHMVR KILNLRVFED ESGKHWSKSV MDKQYEILCV SQFTLQCVLK GNKPDFHLAM PTEQAEGFYN SFLEQLRKTY RPELIKDGKF GAYMQVHIQN DGPVTIELES PAPGTATSDP KQLSKLEKQQ QRKEKTRAKG PSESSKERNT PRKEDRSASS GAEGDVSSER EP
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: DTD1 D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: DTD1
• Synonyms: DUEB; HARS2; pqn-68; C20orf88; bA379J5.3; bA555E18.1
• Gene ID: 92675
• mRNA Refseq: NM_080820.4
• Protein Refseq: NP_543010.3
• MIM: 610996
• UniProt ID: Q8TEA8
• Chromosome Location: 20p11.23
• Function: hydrolase activity, acting on ester bonds