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Recombinant Human D-tyrosyl-tRNA deacylase 1, His-tagged

Cat.No.: DTD1-392H
Product Overview: DTD1, 1-209aa, Human, His tag, E.coli
Description: DTD1 hydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr). It could be a defense mechanism against a harmful effect of D-tyrosine. DTD1 may be a risk factor for aspirin-intolerant asthma (AIA) by catalyzing the hydrolysis of D-tryptophan and interacting with the tyrosyl-tRNA synthetase (tyrRS) enzyme, which promotes a pro-inflammatory phenotype. This protein is localize to the DUE(DNA unwinding element )s of active replication origins. Recombinant human DTD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Source: E.coli
Species: Human
Tag: His-tag
Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
Molecular Mass: 25.9kDa (232aa) confirmed by MALDI-TOF
AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMKAVVQR VTRASVTVGG EQISAIGRGI CVLLGISLED TQKELEHMVR KILNLRVFED ESGKHWSKSV MDKQYEILCV SQFTLQCVLK GNKPDFHLAM PTEQAEGFYN SFLEQLRKTY RPELIKDGKF GAYMQVHIQN DGPVTIELES PAPGTATSDP KQLSKLEKQQ QRKEKTRAKG PSESSKERNT PRKEDRSASS GAEGDVSSER EP
Purity: >85% by SDS - PAGE
Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Concentration: 0.5 mg/ml (determined by Bradford assay)
Gene Name: DTD1 D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol: DTD1
Synonyms: DUEB; HARS2; pqn-68; C20orf88; bA379J5.3; bA555E18.1
Gene ID: 92675
mRNA Refseq: NM_080820.4
Protein Refseq: NP_543010.3
MIM: 610996
UniProt ID: Q8TEA8
Chromosome Location: 20p11.23
Function: hydrolase activity, acting on ester bonds;

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