Recombinant Human epoxide hydrolase 1, microsomal (xenobiotic), T7 tagged

• Cat.No.: EPHX1-463H
• Product Overview: EPHX1, 21- 455aa Human, T7-tagged, E.coli

Specification

• Description: Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Recombinant human EPHX1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Source: E.coli
• Species: Human
• Tag: T7 tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea
• Molecular Mass: 52.2 kDa (451aa)
• AA Sequence: MASMTGGQQM GRGSHMRDKE ETLPLEDGWW GPGTRSAARE DDSIRPFKVE TSDEEIHDLH QRIDKFRFTP PLEDSCFHYG FNSNYLKKVI SYWRNEFDWK KQVEILNRYP HFKTKIEGLD IHFIHVKPPQ LPAGHTPKPL LMVHGWPGSF YEFYKIIPLL TDPKNHGLSD EHVFEVICPS IPGYGFSEAS SKKGFNSVAT ARIFYKLMLR LGFQEFYIQG GDWGSLICTN MAQLVPSHVK GLHLNMALVL SNFSTLTLLL GQRFGRFLGL TERDVELLYP VKEKVFYSLM RESGYMHIQC TKPDTVGSAL NDSPVGLAAY ILEKFSTWTN TEFRYLEDGG LERKFSLDDL LTNVMLYWTT GTIISSQRFY KENLGQGWMT QKHERMKVYV PTGFSAFPFE LLHTPEKWVR FKYPKLISYS YMVRGGHFAA FEEPELLAQD IRKFLSVLER Q
• Purity: >90 % by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: EPHX1 epoxide hydrolase 1, microsomal (xenobiotic) [ Homo sapiens ]
• Official Symbol: EPHX1
• Synonyms: EPHX1; epoxide hydrolase 1, microsomal (xenobiotic); EPHX; epoxide hydrolase 1; epoxide hydratase; MEH; EPOX; HYL1
• Gene ID: 2052
• mRNA Refseq: NM_000120
• Protein Refseq: NP_000111
• UniProt ID: P07099
• Chromosome Location: 1q42.1
• Pathway: Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Metabolism of xenobiotics by cytochrome P450, organism-specific biosystem; Metabolism of xenobiotics by cytochrome P450, conserved biosystem
• Function: cis-stilbene-oxide hydrolase activity; epoxide hydrolase activity; hydrolase activity