Recombinant Human spindle and kinetochore associated complex subunit 1, His-tagged

• Cat.No.: SKA1-499H
• Product Overview: SKA1, 1-255aa Human, His tag, E.coli

Specification

• Description: SKA1, as known as spindle and kinetochore-associated protein 1, is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules. Recombinant human SKA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl
• Molecular Mass: 31.9 kDa(278aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMASSDLE QLCSHVNEKI GNIKKTLSLR NCGQEPTLKT VLNKIGDEII VINELLNKLE LEIQYQEQTN NSLKELCESL EEDYKDIEHL KENVPSHLPQ VTVTQSCVKG SDLDPEEPIK VEEPEPVKKP PKEQRSIKEM PFITCDEFNG VPSYMKSRLT YNQINDVIKE INKAVISKYK ILHQPKKSMN SVTRNLYHRF IDEETKDTKG RYFIVEADIK EFTTLKADKK FHVLLNILRH CRRLSEVRGG GLTRYVIT
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: SKA1 spindle and kinetochore associated complex subunit 1 [ Homo sapiens ]
• Official Symbol: SKA1
• Synonyms: SKA1; spindle and kinetochore associated complex subunit 1; C18orf24, chromosome 18 open reading frame 24; spindle and kinetochore-associated protein 1; MGC10200; spindle and KT (kinetochore) associated 1; C18orf24
• Gene ID: 220134
• mRNA Refseq: NM_001039535
• Protein Refseq: NP_001034624
• UniProt ID: Q96BD8
• Chromosome Location: 18q21.1
• Pathway: Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; DNA Replication, organism-specific biosystem; M Phase, organism-specific biosystem; Mitotic M-M/G1 phases, organism-specific biosystem; Mitotic Prometaphase, organism-specific biosystem
• Function: microtubule binding; protein binding