Recombinant Ovine Growth Hormone, Antagonist
Cat.No. : | GH-36O |
Product Overview : | Recombinant Ovine Somatotropin Antagonist G119R produced inE.Coliis a single, non-glycosylated polypeptide chain containing 191 amino acids and having a molecular mass of 22 kDa. Recombinant Ovine Somatotropin Antagonist is purified by proprietary chromatographic techniques. |
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- Gene Information
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Cat. No. : | GH-36O |
Description : | GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. |
Source : | Escherichia Coli. |
Amino Acid Sequence : | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala. |
Physical Appearance : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity : | Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : | The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045 mM NaHCO3. |
Solubility : | It is recommended to reconstitute the lyophilized GH G119R in 0.4% NaHCO3or water adjusted to pH 9, not less than 100 µg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar. |
Specific Activity : | GH G119R acts as antagonist using an in vitro bioassay in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. It is capable of forming a 1:1 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD) and binds to this ECD with affinity similar the the wild type oGH. |
Protein Content : | Protein quantitation was carried out by: UV spectroscopy at 280 nm using the absorbency value of 0.66 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). |
Stability : | Lyophilized GH G119R although stable at room temperature for at least two weeks, should be stored desiccated below -18°C. Upon reconstitution and filter sterilization GH can be stored at 4°C, pH 9 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Gene Name : | GH growth hormone [ Ovis aries ] |
Synonyms : | GH; growth hormone; GH1; somatotropin |
Gene ID : | 443329 |
mRNA Refseq : | NM_001009315 |
Protein Refseq : | NP_001009315 |
UniProt ID : | P67930 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionIn the treatment of certain diseases, such as growth hormone deficiency and dwarfism, replacement therapy can be achieved with the use of GH protein or its analogues to promote the growth and development of the body.
Studying the regulation of gene expression of GH protein can be achieved through the study of gene transcription, post-transcriptional modification, and translation. For example, it can be done by detecting the expression and activity of transcription factors, or by studying the effects of epigenetic modifications on GH gene expression.
There are complex interactions between GH protein and other hormones. For example, GH protein can stimulate the secretion of insulin-like growth factor-1 (IGF-1), which in turn regulates GH secretion. In addition, GH can also interact with sex hormones to affect the body's growth and metabolic processes.
In clinical practice, the diagnosis of diseases such as growth hormone deficiency, gigantism, and acromegaly can be guided by detecting the level or gene sequence of GH protein, as well as stimulation tests on GH protein. At the same time, based on the structural and functional characteristics of GH proteins, new drugs can be developed to treat these diseases.
The diagnosis of growth hormone deficiency requires a series of laboratory tests, including GH stimulation test, insulin tolerance test, and serum insulin-like growth factor-1 (IGF-1) level. These tests can help your doctor determine whether you have a growth hormone deficiency.
The structure and function of GH proteins are closely related. Its specific amino acid sequence and spatial conformation determine its ability to bind to receptors and activate receptors. Therefore, the understanding of the structure and function of GH protein can help in the development of new drug designs and treatments.
Customer Reviews (3)
Write a reviewDue to its high activity, the experiment can be successful in the first time, reducing the number of experiments.
Fluorescent labeling works well and helps with imaging studies.
The high yield and high purity facilitate our large-scale experiments.
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