Recombinant Human gastric inhibitory polypeptide, His-tagged

• Cat.No.: GIP-546H
• Product Overview: GIP, 22-153aa, Human His tag, E.coli

Specification

• Description: GIP, also known as glucose-dependent insulinotropic polypeptide, is an important insulin-releasing hormone of the enteroinsular axis that has a functional profile of possible therapeutic value for type 2 diabetes. This protein is an important incretin hormone released into the circulation from endocrine K-cells of the duodenum and jejunum after ingestion of food1. It was evaluated for their ability to elevate cellular cAMP production and stimulate insulin secretion. It also promotes plasma triglyceride clearance in response to oral fat loading. In liver, GIP has been shown to enhance insulin-dependent inhibition of glycogenolysis. Recombinant human GIP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 50% glycerol 0.1M NaCl, 2mM DTT
• Molecular Mass: 17.3 kDa(155aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSEKKEGHF SALPSLPVGS HAKVSSPQPR GPRYAEGTFI SDYSIAMDKI HQQDFVNWLL AQKGKKNDWK HNITQREARA LELAGQANRK EEEAVEPQSS PAKNPSDEDL LRDLLIQELL ACLLDQTNLC RLRSR
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: GIP gastric inhibitory polypeptide [ Homo sapiens ]
• Official Symbol: GIP
• Synonyms: GIP; gastric inhibitory polypeptide; glucose dependent insulinotropic polypeptide; glucose-dependent insulinotropic polypeptide
• Gene ID: 2695
• mRNA Refseq: NM_004123
• Protein Refseq: NP_004114
• MIM: 137240
• UniProt ID: P09681
• Chromosome Location: 17q21.3-q22
• Pathway: Class B/2 (Secretin family receptors), organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Glucagon-type ligand receptors, organism-specific biosystem; Incretin Synthesis, Secretion, and Inactivation, organism-specific biosystem; Integration of energy metabolism, organism-specific biosystem
• Function: hormone activity