Recombinant Human U2 small nuclear RNA auxiliary factor 1, His-tagged

• Cat.No.: U2AF1-580H
• Product Overview: U2AF1, 1-240aa , Human, His tag, E.coli

Specification

• Description: U2AF1 belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This protein is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Recombinant human U2AF1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M UREA, 10% glycerol
• Molecular Mass: 30.3 kDa (263aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEYLAS IFGTEKDKVN CSFYFKIGAC RHGDRCSRLH NKPTFSQTIL IQNIYRNPQN SAQTADGSHC AVSDVEMQEH YDEFFEEVFT EMEEKYGEVE EMNVCDNLGD HLVGNVYVKF RREEDAEKAV IDLNNRWFNG QPIHAELSPV TDFREACCRQ YEMGECTRGG FCNFMHLKPI SRELRRELYG RRRKKHRSRS RSRERRSRSR DRGRGGGGGG GGGGGGRERD RRRSRDRERS GRF
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: U2AF1 U2 small nuclear RNA auxiliary factor 1 [ Homo sapiens ]
• Official Symbol: U2AF1
• Synonyms: U2AF1; U2 small nuclear RNA auxiliary factor 1; U2(RNU2) small nuclear RNA auxiliary factor 1 , U2(RNU2) small nuclear RNA auxiliary factor binding protein , U2AFBP; splicing factor U2AF 35 kDa subunit; RN; RNU2AF1; U2AF35; splicing factor U2AF 35kDa subunit; U2 small nuclear RNA auxillary factor 1; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor binding protein; U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit; FP793; U2AFBP; DKFZp313J1712
• Gene ID: 7307
• mRNA Refseq: NM_001025203
• Protein Refseq: NP_001020374
• MIM: 191317
• UniProt ID: Q01081
• Chromosome Location: 21q22.3
• Pathway: Cleavage of Growing Transcript in the Termination Region, organism-specific biosystem; Gene Expression, organism-specific biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; RNA Polymerase II Transcription, organism-specific biosystem; RNA Polymerase II Transcription Termination, organism-specific biosystem; Shigellosis, organism-specific biosystem; Spliceosome, organism-specific biosystem
• Function: RNA binding; RS domain binding; metal ion binding; nucleotide binding; protein binding; zinc ion binding