| Description : |
Mammalian mitochondrial ribosomal proteins help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 6p. Recombinant human MRPL48 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Source : |
E.coli |
| Species : |
Human |
| Tag : |
His-tag |
| Form : |
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol |
| Molecular Mass : |
23.1 kDa (207aa) |
| AA Sequence : |
MGSSHHHHHH SSGLVPRGSH MGSSGEKPIY SVGGILLSIS RPYKTKPTHG IGKYKHLIKA EEPKKKKGKV EVRAINLGTD YEYGVLNIHL TAYDMTLAES YAQYVHNLCN SLSIKVEESY AMPTKTIEVL QLQDQGSKML LDSVLTTHER VVQISGLSAT FAEIFLEIIQ SSLPEGVRLS VKEHTEEDFK GRFKARPELE ELLAKLK |
| Purity : |
>85% by SDS - PAGE |
| Storage : |
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| Concentration : |
1 mg/ml (determined by Bradford assay) |
