Recombinant Human glucosidase, beta, acid 3, His-tagged

• Cat.No.: GBA3-626H
• Product Overview: GBA3, 1-469aa, Human, His tag, E.coli

Specification

• Description: GBA3 is an enzyme that can hydrolyze several types of glycosides. Some individuals, as represented by the reference genome allele, contain a single nucleotide polymorphism that results in a premature stop codon in the coding region, and therefore this allele is pseudogenic due to the failure to produce a functional full-length protein. Recombinant human GBA3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 1X Phosphate Buffered Saline (pH7.4) containing 20% glycerol, 1mM DTT
• Molecular Mass: 56.1 kDa (492aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAFPAGF GWAAATAAYQ VEGGWDADGK GPCVWDTFTH QGGERVFKNQ TGDVACGSYT LWEEDLKCIK QLGLTHYRFS LSWSRLLPDG TTGFINQKGI DYYNKIIDDL LKNGVTPIVT LYHFDLPQTL EDQGGWLSEA IIESFDKYAQ FCFSTFGDRV KQWITINEAN VLSVMSYDLG MFPPGIPHFG TGGYQAAHNL IKAHARSWHS YDSLFRKKQK GMVSLSLFAV WLEPADPNSV SDQEAAKRAI TFHLDLFAKP IFIDGDYPEV VKSQIASMSQ KQGYPSSRLP EFTEEEKKMI KGTADFFAVQ YYTTRLIKYQ ENKKGELGIL QDAEIEFFPD PSWKNVDWIY VVPWGVCKLL KYIKDTYNNP VIYITENGFP QSDPAPLDDT QRWEYFRQTF QELFKAIQLD KVNLQVYCAW SLLDNFEWNQ GYSSRFGLFH VDFEDPARPR VPYTSAKEYA KIIRNNGLEA HL
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: GBA3 glucosidase, beta, acid 3 (cytosolic) [ Homo sapiens ]
• Official Symbol: GBA3
• Synonyms: GBA3; glucosidase, beta, acid 3 (cytosolic); cytosolic beta-glucosidase; GLUC; klotho related protein; KLrP; klotho-related protein; cytosolic beta-glucosidase-like protein 1; CBGL1; MGC104276; MGC126878
• Gene ID: 57733
• mRNA Refseq: NM_001128432
• Protein Refseq: NP_001121904
• MIM: 606619
• UniProt ID: Q9H227
• Chromosome Location: 4p15.31
• Pathway: Cyanoamino acid metabolism, organism-specific biosystem; Cyanoamino acid metabolism, conserved biosystem; Glycosphingolipid metabolism, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Sphingolipid metabolism, organism-specific biosystem
• Function: beta-galactosidase activity; beta-glucosidase activity; cation binding; glycosylceramidase activity