Source : |
HEK293 |
Species : |
Canine |
Tag : |
mFc |
Form : |
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Bio-activity : |
Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 5-30 ng/mL. |
Molecular Mass : |
The recombinant canine FGF9 comprises 441 amino acids and has a predicted molecular mass of 49.8 kDa. The apparent molecular mass of the protein is approximately 50 kDa in SDS-PAGE under reducing conditions. |
Proteinlength : |
Leu4-Ser208 |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
Purity : |
> 75 % as determined by SDS-PAGE |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |