||Recombinant murine FGF-23 is a 25.5 kDa globular protein containing 228 amino acid residues.
||This gene encodes a member of the fibroblast growth factor family. The encoded protein regulates phosphate homeostasis and vitamin D metabolism. Mutation of the related gene in humans causes autosomal dominant hypophosphatemic rickets (ADHR). The secreted protein is further cleaved into N- and C-terminal chains, which results in loss of function.
||Determined by its ability to stimulate the proliferation of murine NIH-3T3 cells. The expected ED50 for this effect is 0.3-0.5 μg/ml, in the presence of murine Klotho and heparin.
||MYPDTSPLLG SNWGSLTHLY TATARTSYHL QIHRDGHVDG TPHQTIYSAL MITSEDAGSV VITGAMTRRF LCMDLHGNIF GSLHFSPENC KFRQWTLENG YDVYLSQKHH YLVSLGRAKR IFQPGTNPPP FSQFLARRNE VPLLHFYTVR PRRHTRSAED PPERDPLNVL KPRPRATPVP VSCSRELPSA EEGGPAASDP LGVLRRGRGD ARGGAGGADR CRPFPRFV
||Greater than 95% by SDS-PAGE gel and HPLC analyses.