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Recombinant Bovine ACTA1, His-tagged

Cat.No. : ACTA1-3090B
Product Overview : Actin, alpha skeletal muscle (ACTA1)
  • Specification
  • Gene Information
  • Related Products
Source : E. Coli or Yeast
Species : Bovine
Tag : His
Form : This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
Protein length : 377
Purity : >90%
Notes : Small volumes of ACTA1 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
Storage : Store at -20 degree C. For extended storage, store at -20 or -80 degree C.
Storage Buffer : PBS pH 7.4, 50% glycerol
Warning : This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Gene Name : ACTA1 actin, alpha 1, skeletal muscle [ Bos taurus ]
Official Symbol : ACTA1
Synonyms : ACTA1; actin, alpha skeletal muscle; alpha-actin-1
Gene ID : 281592
mRNA Refseq : NM_174225
Protein Refseq : NP_776650
UniProt ID : P68138
Chromosome Location : chromosome: 28
Pathway : Striated Muscle Contraction, organism-specific biosystem
Function : ATP binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (20)

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What were the findings when transgenic mice expressing ACTA1(D286G) were created? 12/04/2022

The Tg(ACTA1)(D286G) mice exhibited decreased activity levels, weaker skeletal muscles, and pathological lesions similar to those observed in human patients. However, unlike the patients, the mice had a normal lifespan.

What is the function of the ACTA1 protein? 07/11/2022

The ACTA1 protein, also known as skeletal muscle α-actin, plays a crucial role in muscle contraction and the maintenance of muscle structure. It is a key component of the sarcomeres, the structural units of muscle fibers.

What significance do the ringbinden fibers have in relation to ACTA1 mutations? 04/10/2022

The presence of ringbinden fibers in the mouse models suggests that patients with ringbinden fibers of unknown genetic origin should be considered for ACTA1 mutation screening, indicating a possible link between these features and ACTA1 mutations.

How is ACTA1 inhibited by PAX3-FOXO1 in ARMS? 01/25/2022

ACTA1 is inhibited by PAX3-FOXO1 both at the transcriptional level and the protein level. This inhibition occurs through the RhoA-MKL1-SRF signaling pathway.

What techniques can be used to study the ACTA1 protein? 12/18/2021

Researchers employ various techniques to study the ACTA1 protein, such as immunohistochemistry, immunoblotting, and mass spectrometry. These methods help identify the presence, abundance, and localization of ACTA1 in muscle tissues or cells.

How do mutations in the ACTA1 gene contribute to Nemaline myopathy (NM)? 06/06/2021

Mutations in the ACTA1 gene account for a significant proportion of NM cases. Most of these mutations are missense mutations, which cause autosomal dominant NM by producing an abnormal protein. However, about 10% of ACTA1 gene mutations are associated with autosomal recessive NM, resulting in loss of protein function.

Are there any known interacting proteins or partners of ACTA1? 01/13/2021

Yes, the ACTA1 protein interacts with other proteins involved in muscle function and structure. For example, it forms complexes with other actin-binding proteins, such as myosin and tropomyosin, to regulate muscle contraction.

What is the relationship between mutations in the ACTA1 gene and congenital myopathies? 08/06/2020

Mutations in the skeletal muscle α-actin gene (ACTA1) cause various congenital myopathies, resulting in severe skeletal muscle weakness in most patients.

What is the difference in inheritance patterns between dominant and recessive ACTA1 gene mutations? 05/23/2020

The majority of ACTA1 gene mutations cause autosomal dominant NM, meaning a single abnormal copy of the gene is sufficient to cause the disorder. In contrast, approximately 10% of ACTA1 gene mutations lead to autosomal recessive NM, requiring two copies of the mutated gene (one from each parent) to manifest the disorder.

How does the RhoA-MKL1-SRF signaling pathway impact the inhibition of ACTA1? 04/03/2020

The RhoA-MKL1-SRF signaling pathway is involved in mediating the inhibition of ACTA1 by PAX3-FOXO1. It is a signaling cascade that transmits the inhibitory signals from PAX3-FOXO1 to downregulate the expression of ACTA1.

 How is the ACTA1 protein normally regulated in skeletal muscle? 03/12/2020

The expression and regulation of the ACTA1 protein in skeletal muscle are tightly controlled by various molecular mechanisms, including transcriptional and post-transcriptional processes. These mechanisms ensure proper levels of ACTA1 for muscle function.

What protein composition was observed in the skeletal muscles of Tg(ACTA1)(D286G) mice? 02/10/2020

Mass spectrometry analysis revealed that the skeletal muscles of Tg(ACTA1)(D286G) mice contained approximately 25% of the ACTA1(D286G) protein.

How do ACTA1 mutations affect muscle structure? 01/29/2020

CTA1 mutations can disrupt the normal organization of muscle fibers and sarcomeres, leading to structural abnormalities. These abnormalities can include the presence of nemaline bodies, actin accumulations, and widespread sarcomeric disarray.

What happens when there are mutations in the ACTA1 gene? 12/07/2019

Mutations in the ACTA1 gene can lead to various congenital myopathies, which are muscle disorders present from birth or early infancy. These mutations can disrupt the normal function and structure of skeletal muscles.

What are some of the consequences of ACTA1 mutations? 07/21/2018

ACTA1 mutations can result in severe skeletal muscle weakness, leading to difficulties in movement and muscle function. In some cases, the severity of the mutations can be life-threatening, causing reduced lifespan and early mortality, as seen in certain dominant ACTA1 mutations.

Are there different types of ACTA1 mutations? 04/08/2018

 Yes, there are various types of ACTA1 mutations identified in patients with congenital myopathies. These mutations can have different effects on the structure and function of the ACTA1 protein, leading to a spectrum of clinical presentations and disease severity.

 What is the potential therapeutic implication related to ACTA1 protein load? 04/19/2017

Studies have suggested that altering the ratio of mutant ACTA1 protein to wild-type actin protein in patient muscles may hold promise as a potential therapy for patients with dominant ACTA1 disease

Where is the ACTA1 protein primarily found? 02/06/2017

The ACTA1 protein is primarily found in skeletal muscle, which is responsible for voluntary muscle movement in the body.

What is the significance of the inhibition of ACTA1 by PAX3-FOXO1? 01/06/2016

The inhibition of ACTA1 by PAX3-FOXO1 is believed to play a role in the tumorigenesis and development of alveolar rhabdomyosarcoma (ARMS). It suggests that this regulatory mechanism contributes to the disease progression.

What potential therapy is suggested based on the correlation between mutant ACTA1 protein load and disease severity? 01/02/2016

Altering the ratio of mutant ACTA1 protein to wild-type actin in patient muscle may be a potential therapy for patients with dominant ACTA1 disease.

Customer Reviews (5)

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Reviews
12/25/2021

    I found the protein product to be highly stable, retaining its functionality even after months of storage, which was crucial for my long-term experiments

    05/11/2021

      The protein product's reliable performance and consistent quality reassured me that it would not introduce any confounding factors or jeopardize the integrity of my experiments.

      04/24/2018

        I found the protein product to be compatible with various downstream applications, such as Western blotting and ELISA, broadening its utility in my researc

        07/12/2017

          I appreciated the convenient packaging of the protein product, with individual aliquots that reduced the need for repeated thawing and freezing.

          11/12/2016

            I appreciated the protein product's consistent performance at the specified concentrations, enabling me to achieve accurate dosing in my experiments

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