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Active Recombinant Human GBA, MYC/DDK-tagged

Cat.No. : GBA-020H
Product Overview : Recombinant Human GBA, fused with C-terminal MYC/DDK, was expressed in HEK293 cells.
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  • Gene Information
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Description : This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants.
Source : HEK293 cells
Species : Human
Tag : MYC/DDK
Bio-activity : The enzymatic activity of GBA was measured by its ability to hydrolyze a fluorescent substrate 4-methylumbelliferyl-ß-D-glucopyranoside. The specific activity is > 70,000 pmol/hour/µg, as measured under the following conditions: 27 ng of GBA was incubated with 10 mM 4-methylumbelliferyl- ß-D-glucopyranoside in the following buffer at 37°C for 40 min: 150 mM citrate-phosphate buffer, pH 5.4, 0.25% (w/w) sodium taurocholate, 0.25% (w/w) Triton X-100, and 1% bovine serum albumin. The reaction was terminated by adding 0.5 volume of 1M glycine buffer, pH 12.5. The hydrolyzed product of reaction, 4-methylumbelliferone (4-MU), was measured using a FlexStation 3 microplate reader (Ex365/Em445). Specific activity of GBA was calculated based on a standard curve of known concentration of 4-MU.
Molecular Mass : 55.5 kDa
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration : >50 ug/mL as determined by microplate BCA method
Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Gene Name : GBA glucosidase, beta, acid [ Homo sapiens ]
Official Symbol : GBA
Synonyms : GBA; GCB; GBA1; GLUC; glucosidase, beta, acid; glucosylceramidase; beta-GC; alglucerase; imiglucerase; acid beta-glucosidase; beta-glucocerebrosidase; lysosomal glucocerebrosidase; D-glucosyl-N-acylsphingosine glucohydrolase; NP_000148.2; EC 3.2.1.45; NP_001005741.1; NP_001005742.1; NP_001165282.1; NP_001165283.1
Gene ID : 2629
mRNA Refseq : NM_000157
Protein Refseq : NP_000148
MIM : 606463
UniProt ID : P04062
Chromosome Location : 1q21
Pathway : Glycosphingolipid metabolism; Other glycan degradation; Sphingolipid metabolism
Function : glucosylceramidase activity; protein binding; receptor binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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06/08/2022

    This guidance allows me to design and implement experiments effectively while adhering to best practices, enhancing the reliability and reproducibility of my results.

    08/08/2021

      They ensure the purity, integrity, and functionality of GBA protein through rigorous quality control measures.

      05/02/2018

        The manufacturer's commitment to quality control is essential in achieving reliable and consistent outcomes

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