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Active Recombinant Human SIRT3, His-tagged

Cat.No. : SIRT3-346H
Product Overview : Recombinant Human SIRT3(Ser101-Lys399), fused with an N-terminal Met and 6-His tag, was expressed in E. coli.
  • Specification
  • Gene Information
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Description : This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.
Source : E. coli
Species : Human
Tag : His
Predicted N Terminal : Met
Form : Supplied as a 0.2 µm filtered solution in Tris, NaCl, Brij-35 and Glycerol.
Bio-activity : Measured by its ability to remove the acetyl group from a fluorogenic peptide substrate Ac-RGK(Ac)-AMC in a coupled assay.
Molecular Mass : Predicted Molecular Mass: 34 kDaSDS-PAGE: 30-33 kDa, reducing conditions
Endotoxin : < 1.0 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage : Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 °C as supplied.3 months, -20 to -70 °C under sterile conditions after opening.
Gene Name : SIRT3 sirtuin 3 [ Homo sapiens (human) ]
Official Symbol : SIRT3
Synonyms : SIRT3; sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae); SIR2L3; NAD-dependent deacetylase sirtuin-3, mitochondrial; EC 3.5.1.-; SIR2-like protein 3; hSIRT3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase 2; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3; sirtuin 3; sirtuin type 3
Gene ID : 23410
mRNA Refseq : NM_001017524
Protein Refseq : NP_001017524
MIM : 604481
UniProt ID : Q9NTG7
Chromosome Location : 11p15.5
Pathway : Energy Metabolism; Mitochondrial biogenesis; Organelle biogenesis and maintenance
Function : NOT NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity (H3-K14 specific); protein binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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