Active Recombinant Human CD40LG, MIgG2a Fc-tagged

• Cat.No.: CD40LG-34H
• Product Overview: The extracellular domain of human CD40L (NP_000065.1) (Met113-Leu261) is fused to the N-terminus of the Fc portion of mouse IgG2a was expressed in CHO celL

Specification

• Description: The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
• Source: CHO cells
• Species: Human
• Tag: MIgG2a Fc-tagged
• Form: Lyophilized from 0.2μm-filtered solution in PBS.
• Bio-activity: Measured in a cell proliferation assay using enriched human B cells in the presence of IL-4.
• Molecular Mass: 46 KDa (monomer)
• AA Sequence: Met113-Leu261
• Endotoxin: <0.06 eu/μg as determined by lal>
• Purity: >98%, by SDS-PAGE under reducing conditions.
• Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
• Reconstitution: Reconstitute at 100μg/ml in sterile PBS.
• Warning: Avoid freeze/thaw cycles.

Gene Information

• Gene Name: CD40LG CD40 ligand [ Homo sapiens ]
• Official Symbol: CD40LG
• Synonyms: CD40LG; CD40 ligand; HIGM1, IMD3, TNFSF5, tumor necrosis factor (ligand) superfamily, member 5 (hyper IgM syndrome); CD40 antigen ligand; CD40L; CD154; gp39; hCD40L; hyper IgM syndrome; T B cell activating molecule; TNF related activation protein; TRAP; tumor necrosis factor (ligand) superfamily member 5; CD40-L; T-cell antigen Gp39; T-B cell-activating molecule; TNF-related activation protein; IGM; IMD3; HIGM1; T-BAM; TNFSF5
• Gene ID: 959
• mRNA Refseq: NM_000074
• Protein Refseq: NP_000065
• MIM: 300386
• UniProt ID: P29965
• Chromosome Location: Xq26
• Pathway: Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem
• Function: CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding