| Description : |
The gene IL12B encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children and the gene IL12A encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. |
| Source : |
CHO cell |
| Species : |
Human |
| Tag : |
HIgG1 Fc-tagged |
| Form : |
Lyophilized from 0.2μm-filtered solution in PBS. |
| Bio-activity : |
Measured in a cell proliferation assay. |
| Molecular Mass : |
85KDa |
| AA Sequence : |
The human IL-12 complex composed of the p40 subunit (NP_002178.2) (ILe23-Ser328) and the p35 subunit (NP_000873.2)(Arg57-Ser253) is fused through a polypeptide linker to the Fc region of human IgG1. |
| Endotoxin : |
<0.06 eu/μg as determined by lal test.> |
| Purity : |
≥98%, by SDS-PAGE under reducing conditions. |
| Stability : |
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Reconstitution : |
Reconstitute at 100μg/ml in sterile PBS. |
| Warning : |
Avoid freeze/thaw cycles. |
