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Recombinant Human FCGR2A cell lysate

Cat.No. : FCGR2A-001HCL
Product Overview : Human CD32a / FCGR2A (167 His) derived in CHO cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : CHO cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : FCGR2A Fc fragment of IgG, low affinity IIa, receptor (CD32) [ Homo sapiens ]
Official Symbol : FCGR2A
Synonyms : FCGR2A; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIa, receptor for (CD32) , FCG2, FCGR2, FCGR2A1; low affinity immunoglobulin gamma Fc region receptor II-a; CD32; CD32A; CDw32; IGFR2; Immunoglobulin G Fc receptor II; fcRII-a; fc-gamma-RIIa; fc-gamma RII-a; igG Fc receptor II-a; FCG2; FcGR; FCGR2; FCGR2A1; MGC23887; MGC30032;
Gene ID : 2212
mRNA Refseq : NM_001136219
Protein Refseq : NP_001129691
MIM : 146790
UniProt ID : P12318
Chromosome Location : 1q23
Pathway : Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Leishmaniasis, organism-specific biosystem; Leishmaniasis, conserved biosystem; Osteoclast differentiation, organism-specific biosystem; Osteoclast differentiation, conserved biosystem; Phagosome, organism-specific biosystem;
Function : IgG binding; receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does FCGR2A impact the risk and severity of certain allergic reactions? 03/22/2023

FCGR2A may influence the risk and severity of allergic reactions by modulating the immune response to allergens and antibodies involved in allergic processes.

In the context of infectious diseases, how does FCGR2A contribute to host defense mechanisms? 11/02/2022

FCGR2A participates in host defense against infectious diseases by facilitating the recognition and clearance of pathogens through antibody-mediated immune responses.

How does FCGR2A contribute to the understanding of immunogenetics? 05/26/2021

FCGR2A contributes to the understanding of immunogenetics by highlighting the genetic factors that influence immune responses and the development of immune-related diseases.

How can genetic variations in FCGR2A impact an individual's response to immunotherapy? 03/10/2020

Genetic variations in FCGR2A can influence an individual's response to immunotherapy by affecting the affinity of FCGR2A for antibodies, thereby modulating immune cell activation.

In what ways can FCGR2A be targeted for therapeutic interventions? 12/11/2017

FCGR2A can be targeted for therapeutic interventions through the development of drugs that modulate its activity, offering potential strategies for treating immune-related disorders.

Customer Reviews (3)

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Reviews
03/22/2023

    With its purity and reliability, this protein is poised to contribute significantly to the success of my research objectives by providing accurate and reproducible results.

    02/26/2023

      Its versatility allows for exploration of its extensive involvement in various biochemical and biological processes, providing valuable insights into its role and implications.

      07/24/2017

        The FCGR2A Protein demonstrates remarkable stability and functional efficiency, making it an indispensable tool for diverse scientific applications.

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