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Recombinant Human CPNE1 cell lysate

Cat.No. : CPNE1-001HCL
Product Overview : Human CPNE1 / Copine I / CPN1 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : CPNE1 copine I [ Homo sapiens ]
Official Symbol : CPNE1
Synonyms : CPNE1; copine I; copine-1; CPN1; COPN1; MGC1142;
Gene ID : 8904
mRNA Refseq : NM_001198863
Protein Refseq : NP_001185792
MIM : 604205
UniProt ID : Q99829
Chromosome Location : 20q11.22
Function : calcium-dependent phospholipid binding; phosphatidylserine binding; transporter activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does CPNE1 interact with calcium ions in cellular processes? 12/24/2021

It binds to calcium ions, facilitating its interaction with phospholipids and proteins.

Are there known genetic alterations in CPNE1 associated with specific diseases? 02/26/2021

Genetic alterations in CPNE1 have been associated with various types of cancer.

What role does CPNE1 play in the regulation of neuronal activity? 08/01/2020

In neurons, CPNE1 is believed to play a role in synaptic function and plasticity.

What is the primary biological function of CPNE1? 07/14/2019

CPNE1 is involved in calcium-dependent membrane interactions and cellular signaling.

In which tissues or cell types is CPNE1 predominantly expressed? 04/05/2019

CPNE1 shows a broad expression pattern, including in neural and cancerous tissues.

How is CPNE1 implicated in cancer progression? 12/17/2018

CPNE1 has been found to be upregulated in certain cancers, promoting tumor progression.

What research or therapeutic strategies are being explored involving CPNE1? 05/13/2018

Research is focused on understanding its role in cancer, and it’s being explored as a potential therapeutic target.

Customer Reviews (3)

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Reviews
07/08/2021

    Reliable results, quality product.

    03/15/2021

      Worked great, fast shipping.

      06/27/2020

        Very happy with purchase.

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