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Recombinant Human MAP2K6 cell lysate

Cat.No. : MAP2K6-001HCL
Product Overview : Human MAP2K6 / MKK6 / MEK6 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : MAP2K6 mitogen-activated protein kinase kinase 6 [ Homo sapiens ]
Official Symbol : MAP2K6
Synonyms : MAP2K6; mitogen-activated protein kinase kinase 6; PRKMK6; dual specificity mitogen-activated protein kinase kinase 6; MAPKK6; MEK6; MKK6; protein kinase; mitogen activated; kinase 6 (MAP kinase kinase 6); SAPKK3; MEK 6; MAPKK 6; SAPK kinase 3; MAPK/ERK kinase 6; stress-activated protein kinase kinase 3; protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6); SAPKK-3;
Gene ID : 5608
mRNA Refseq : NM_002758
Protein Refseq : NP_002749
MIM : 601254
UniProt ID : P52564
Chromosome Location : 17q
Pathway : Activated TLR4 signalling, organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem; CDC42 signaling events, organism-specific biosystem; CDO in myogenesis, organism-specific biosystem; CXCR3-mediated signaling events, organism-specific biosystem; Cellular roles of Anthrax toxin, organism-specific biosystem;
Function : ATP binding; MAP kinase kinase activity; nucleotide binding; protein kinase binding; protein serine/threonine kinase activity; protein tyrosine kinase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Is MAP2K6 a potential biomarker for predicting treatment response? 05/02/2022

Its expression levels in certain cancers are being explored as predictive biomarkers to anticipate response to specific therapies.

Is MAP2K6 involved in any other diseases besides cancer? 04/15/2021

Studies also suggest its involvement in cardiovascular diseases and neurological disorders.

How does MAP2K6 relate to drug resistance in cancer treatment? 12/06/2020

MAP2K6 signaling pathways might play a role in drug resistance mechanisms, making it a focus for overcoming therapeutic challenges.

Are there any known diseases associated with MAP2K6 mutations? 11/01/2019

Yes, mutations in MAP2K6 have been linked to certain cancers, including colorectal cancer and lung adenocarcinoma.

Can MAP2K6 inhibition lead to potential side effects? 06/26/2018

As with any targeted therapy, MAP2K6 inhibitors might have side effects, which are under investigation in preclinical and clinical studies.

Customer Reviews (3)

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Reviews
07/21/2022

    I am certain that the MAP2K6 protein will play a pivotal role in facilitating robust and insightful research, providing the assurance of reliable and consistent results.

    03/23/2022

      The excellent technical support provided by the manufacturer can solve my problem.

      12/27/2021

        Whether I am studying protein-protein interactions, investigating cellular pathways, or examining protein folding dynamics, the MAP2K6 protein consistently delivers exceptional performance and precise outcomes.

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