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Recombinant Human RPS6KA2 cell lysate

Cat.No. : RPS6KA2-610HCL
Product Overview : Human RPS6KA2 / RSK3 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : RPS6KA2 ribosomal protein S6 kinase, 90kDa, polypeptide 2 [ Homo sapiens ]
Official Symbol : RPS6KA2
Synonyms : RPS6KA2; ribosomal protein S6 kinase, 90kDa, polypeptide 2; ribosomal protein S6 kinase, 90kD, polypeptide 2; ribosomal protein S6 kinase alpha-2; HU 2; RSK; RSK3; RSK-3; p90RSK2; p90-RSK 2; MAPKAPK-1c; S6K-alpha-2; MAPKAP kinase 1c; ribosomal S6 kinase 3; MAPK-activated protein kinase 1c; ribosomal protein S6 kinase alpha 2; 90 kDa ribosomal protein S6 kinase 2; MAP kinase-activated protein kinase 1c; HU-2; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2;
Gene ID : 6196
mRNA Refseq : NM_001006932
Protein Refseq : NP_001006933
MIM : 601685
UniProt ID : Q15349
Chromosome Location : 6q27
Pathway : Activated TLR4 signalling, organism-specific biosystem; Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Axon guidance, organism-specific biosystem; CREB phosphorylation, organism-specific biosystem; CREB phosphorylation through the activation of Ras, organism-specific biosystem; Cytoplasmic Ribosomal Proteins, organism-specific biosystem; Developmental Biology, organism-specific biosystem;
Function : ATP binding; magnesium ion binding; nucleotide binding; protein serine/threonine kinase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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