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Recombinant Rat Interleukin 1 beta

Cat. No.: Il1b-93R
Product Overview: Recombinant RatInterleukin-1b produced inE.Coliis a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17415.82 Dalton. The IL-1b is purified by proprietary chromatographic techniques.
Description: Interleukin-1b is produced by activated macrophages, IL-1B stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1B proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Source: Escherichia Coli.
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Pro-Ile-Arg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation: The protein was lyophilized without any additives.
Solubility: It is recommended to reconstitute the lyophilized Interleukin 1b in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Specific Activity: The ED50range= 0.2-0.5 ng/ml, determined by the dose dependent proliferation of mouse D10S cells.
Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.558 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-1b as a Reference Standard.
Stability: Lyophilized Interleukin-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1b should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name: Il1b interleukin 1 beta [ Rattus norvegicus ]
Synonyms: Il1b; interleukin 1 beta;interleukin 1, beta ; IMAGE:7365029; MGC:108658
Gene ID: 24494
mRNA Refseq: NM_031512
Protein Refseq: NP_113700
UniProt ID: Q63264
Chromosome Location: 3q36
Pathway: Alzheimer"s disease; Apoptosis; Cytokine-cytokine receptor interaction; Graft-versus-host disease; Hematopoietic cell lineage; MAPK signaling pathway; Prion diseases; Toll-like receptor signaling pathway; Type I diabetes mellitus
Function: cytokine activity; growth factor activity; interleukin-1 receptor binding; protein domain specific binding

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