Recombinant Mouse LAIR1 cell lysate
Cat.No. : | LAIR1-1331MCL |
Product Overview : | Mouse LAIR1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Lair1 leukocyte-associated Ig-like receptor 1 [ Mus musculus ] |
Official Symbol : | LAIR1 |
Synonyms : | LAIR1; leukocyte-associated Ig-like receptor 1; leukocyte-associated immunoglobulin-like receptor 1; mLAIR1; Lair-1; mLair-1; BB115266; D7Bwg0421e; 5133400O11Rik; |
Gene ID : | 52855 |
mRNA Refseq : | NM_001113474 |
Protein Refseq : | NP_001106945 |
Function : | receptor activity; |
Products Types
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Lair1-1728M | Recombinant Mouse Lair1 Protein, His-tagged | +Inquiry |
LAIR1-166H | Recombinant Human LAIR1 Protein, MYC/DDK-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionLAIR1's role in immune regulation may have implications for reducing immune rejection in regenerative medicine and tissue engineering.
Some clinical trials are exploring the therapeutic potential of targeting LAIR1 in cancer immunotherapy and autoimmune disease management.
LAIR1 can be targeted to enhance the effectiveness of immunotherapy, as inhibiting LAIR1 may boost the immune system's response against cancer cells.
Yes, LAIR1's role in immune modulation makes it relevant in inflammatory conditions. Targeting LAIR1 may be a strategy to manage inflammation.
There are experimental drugs and biologics that target LAIR1 currently in preclinical and early clinical development.
Customer Reviews (3)
Write a reviewAnother advantage of using LAIR1 protein in trials is its potential as a therapeutic agent.
The manufacturer's support and expertise further enhance the utility of LAIR1 in my experimental work, ensuring reliable and high-quality results.
This collaborative partnership greatly contributes to the success of my research projects and facilitates meaningful contributions to the scientific community.
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