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Recombinant Rat Fibroblast Growth Factor 9

Cat. No.: Fgf9-112R
Product Overview: Recombinant Rat FGF9 produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 205 amino acids and having a molecular mass of 23308 Dalton. Recombinant Mous FGF-9 is purified by proprietary chromatographic techniques.
Description: Rat and mouse FGF-9 show a very high homology to human FGF-9. The transcripts for FGF-9 have been found in brain and in kidney tissue. Fibroblast Growth Factor-9 is a member of the fibroblast growth factor (FGF) family. FGF9 was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Fibroblast Growth Factor 9 may have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
Source: Escherichia Coli.
Amino Acid Sequence: PLGEVGSYFG VQDAVPFGNV PVLPVDSPVL LNDHLGQSEA GGLPRGPAVT DLDHLKGILR RRQLYCRTGF HLEIFPNGTI QGTRKDHSRF GILEFISIAV GLVSIRGVDS GLYLGMNEKG ELYGSEKLTQ ECVFREQFEE NWYNTYSSNL YKHVDTGRRY YVALNKDGTP REGTRTKRHQ KFTHFLPRPV DPDKVPELYK DILSQS
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation: The FGF-9 was lyophilized from 10 mM phosphate buffer, pH-7.5 &, 75 mM Ammonium Sulfate.
Solubility: It is recommended to reconstitute the lyophilized Rat FGF-9 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Specific Activity: The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/ml, corresponding to a specific activity of 2 x 106Units/mg.
Stability: Lyophilized Rat Fibroblast Growth Factor-9 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF9 Rat Recombinant should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name: Fgf9 fibroblast growth factor 9 [ Rattus norvegicus ]
Synonyms: Fgf9; fibroblast growth factor 9; IMAGE:7124006; MGC:93094
Gene ID: 25444
mRNA Refseq: NM_012952
Protein Refseq: NP_037084
UniProt ID: P36364
Chromosome Location: p12
Pathway: MAPK signaling pathway; Melanoma; Pathways in cancer; Regulation of actin cytoskeleton
Function: fibroblast growth factor receptor binding; growth factor activity; growth factor activity; heparin binding

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