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Recombinant Mouse IL11RA cell lysate

Cat.No.: IL11RA-2558MCL
Product Overview: Mouse IL11RA / IL11Rα derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
Source: Human cells
Species: Mouse
Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability: Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name: Il11ra1 interleukin 11 receptor, alpha chain 1 [ Mus musculus ]
Official Symbol: Il11ra1
Synonyms: NR1; GP130; Il11ra; Il-11ra; Il11ra2; AI314697; interleukin-11 receptor subunit alpha-1; NR-1; etl-2; locus 2; IL-11RA1; Il-11ra-alpha; IL-11R-alpha-1; IL-11R subunit alpha-1; novel cytokine receptor 1; enhancer trap locus homolog 2; IL-11 receptor subunit alpha-1; interleukin 11 receptor, alpha chain 2
Gene ID: 16157
mRNA Refseq: NM_001163401
Protein Refseq: NP_001156873
UniProt ID: Q64385
Chromosome Location: 4 A5; 4 21.94 cM
Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem
Function: cytokine receptor activity; interleukin-11 binding

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