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Recombinant Human MMP7 cell lysate

Cat.No. : MMP7-2883HCL
Product Overview : Human MMP7 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : MMP7 matrix metallopeptidase 7 (matrilysin, uterine) [ Homo sapiens ]
Official Symbol : MMP7
Synonyms : MMP7; matrix metallopeptidase 7 (matrilysin, uterine); matrix metalloproteinase 7 (matrilysin, uterine) , MPSL1; matrilysin; PUMP 1; matrin; pump-1 protease; uterine matrilysin; uterine metalloproteinase; matrix metalloproteinase-7; matrix metalloproteinase 7 (matrilysin, uterine); MMP-7; MPSL1; PUMP-1;
Gene ID : 4316
mRNA Refseq : NM_002423
Protein Refseq : NP_002414
MIM : 178990
UniProt ID : P09237
Chromosome Location : 11q21-q22
Pathway : Activation of Matrix Metalloproteinases, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Matrix Metalloproteinases, organism-specific biosystem; Syndecan-1-mediated signaling events, organism-specific biosystem; Wnt Signaling Pathway and Pluripotency, organism-specific biosystem;
Function : metal ion binding; metalloendopeptidase activity; peptidase activity; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What clinical conditions are associated with MMP7 overexpression? 01/31/2023

MMP7 overexpression has been linked to various diseases, including cancer, inflammatory disorders, and tissue fibrosis.

In what ways does MMP7 contribute to inflammatory processes? 05/20/2020

MMP7 is involved in the degradation of extracellular matrix components during inflammation, influencing tissue damage and repair processes.

How can MMP7 be targeted for therapeutic interventions? 01/20/2020

Developing specific inhibitors that target MMP7 activity is a potential approach for therapeutic interventions, particularly in diseases where MMP7 plays a detrimental role.

Are there any inhibitors developed for MMP7? 10/19/2017

Several MMP inhibitors have been investigated, and some show promise in controlling MMP7 activity. However, their clinical use is still under research.

How is MMP7 involved in cancer progression? 01/13/2017

MMP7 facilitates cancer cell invasion and metastasis by degrading the extracellular matrix, allowing cancer cells to invade surrounding tissues and spread to distant organs.

Can MMP7 be used as a biomarker for cancer diagnosis? 01/09/2016

Yes, elevated levels of MMP7 have been observed in the serum or tissues of cancer patients, making it a potential biomarker for certain types of cancer.

Customer Reviews (3)

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Reviews
05/01/2023

    This includes rigorous quality control measures to ensure the consistency and purity of the protein, thereby reducing variability in the experimental outcomes.

    05/13/2022

      by utilizing MMP7 protein in trials and collaborating with a supportive manufacturer, researchers can gain access to a versatile tool that can aid in their understanding of angiogenesis and vascular biology.

      05/26/2018

        With expert assistance and reliable product quality, researchers can conduct their experiments with confidence, enabling them to contribute to advancements in the field and potentially improve future therapeutic interventions.

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