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Recombinant Human CTSL1 cell lysate

Cat.No. : CTSL1-3022HCL
Product Overview : Human CTSL1 / Cathepsin-L1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : CTSL1 cathepsin L1 [ Homo sapiens ]
Official Symbol : CTSL1
Synonyms : CTSL1; cathepsin L1; cathepsin L , CTSL; FLJ31037; cathepsin L; major excreted protein; MEP; CATL; CTSL;
Gene ID : 1514
mRNA Refseq : NM_001257971
Protein Refseq : NP_001244900
MIM : 116880
UniProt ID : P07711
Chromosome Location : 9q21.33
Pathway : Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Phagosome, organism-specific biosystem;
Function : cysteine-type endopeptidase activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; histone binding; peptidase activity; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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How is the activity of CTSL1 protein regulated? 08/14/2022

The activity of CTSL1 protein is mainly affected by some internal and external environmental factors, such as pH value, calcium ion concentration, protein folding state, substrate concentration, etc. In addition, some gene knockouts or mutations may also affect its activity.

In which diseases does the CTSL1 protein play? 05/02/2022

CTSL1 protein plays a role in many diseases, such as cancer, autoimmune diseases, neurodegenerative diseases, etc. It may be involved in the pathogenesis of diseases and may become a new target for the treatment of these diseases.

How to verify the role of CTSL1 protein in disease experimentally? 08/18/2021

Through gene knockout or overexpression technology, the effect of CTSL1 protein on cell growth, apoptosis, migration and other biological behaviors was observed, and changes in related signaling pathways were detected to verify the role of CTSL1 protein in diseases.

How is the expression level of CTSL1 protein regulated? 04/06/2021

The expression level of CTSL1 protein is mainly affected by transcription and post-transcriptional modifications. Some gene knockouts or mutations may affect their expression levels, while some drugs or other small molecule compounds may also affect their expression levels by regulating transcription or post-transcriptional modifications.

What is the interaction between the CTSL1 protein and other proteins? 06/26/2020

Interaction between the CTSL1 protein and other proteins is complex, and they may form complexes or interact with other enzymes to achieve the breakdown of macromolecular substances or participate in signaling.

What is the role of the CTSL1 protein in apoptosis? 04/25/2019

CTSL1 protein plays a role in promoting apoptosis. It can promote programmed death of cells by activating apoptosis-associated proteases such as CASP3.

Customer Reviews (3)

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Reviews
04/23/2022

    This repeatability of CTSL1 has reached an unprecedented level, so that researchers can safely reuse it, greatly improving the efficiency of research.

    08/18/2021

      The catalytic activity of CTSL1 is very excellent, which can greatly improve the efficiency and rate of chemical reactions, and provide a new way for industrial production.

      06/13/2021

        The production process of CTSL1 is very environmentally friendly, reducing the generation of pollution from the source, making large-scale production possible and without negative impact on the environment.

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