Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Mouse APCS cell lysate

Cat.No. : APCS-3089MCL
Product Overview : Mouse APCS / SAP derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
  • Related Products
Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Apcs serum amyloid P-component [ Mus musculus ]
Official Symbol : APCS
Synonyms : APCS; serum amyloid P-component; APCS amyloid P-component serum protein; Sap;
Gene ID : 20219
mRNA Refseq : NM_011318
Protein Refseq : NP_035448
Function : metal ion binding; sugar binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (20)

Ask a question
Is there a time limit for therapy? 10/27/2022

The duration of use of therapy depends on the type of disease and treatment regimen, and needs to be carried out under the guidance of a professional physician.

What are the advantages and disadvantages of compared with other treatments? 09/17/2022

The advantages and disadvantages of s versus other treatments need to be evaluated in the context of the specific disease and treatment regimen.

Can s achieve precision medicine? 05/20/2022

The specificity and specificity of s allow them to be used in precision medicine to achieve personalized treatment.

Does the stability of affect its clinical application? 04/08/2022

The stability of the is one of the important factors for its clinical application, and poor stability of the protein may lead to adverse reactions in patients or reduce the therapeutic effect.

Does the have immunogenicity? 04/06/2022

Recombinant proteins, as exogenous proteins, may cause a response from the immune system, but there are already several techniques used to reduce their immunogenicity.

Can s be used in vaccine preparation? 02/20/2022

Recombinant proteins can be used in vaccine preparation, and there are already several vaccines based on s on the market.

Does the work better than traditional drugs? 04/16/2021

The efficacy of s and traditional drugs depends on the type of disease and individual differences, and needs to be selected on a case-by-case basis.

Can s be used to treat what diseases? 03/23/2021

Recombinant proteins can be used to treat a variety of diseases, such as immune deficiency diseases, tumors, cardiovascular diseases, etc.

Can achieve therapeutic effect through oral administration? 01/21/2021

Recombinant proteins usually need to be administered intravenously, but there are also some oral s that are currently being studied.

Is the preparation of capable of large-scale production? 11/30/2020

With the advancement of technology, the preparation of s has also been continuously improved, and large-scale production has become possible.

Is there any potential risk in the application of s? 11/02/2020

Any medical technology and drug treatment has a certain risk, is no exception, need to be reasonable application under the guidance of professional doctors.

Is the preparation of s limited by policies and regulations? 10/19/2020

The preparation of s is subject to compliance with relevant policies and regulations, particularly with regard to biosafety.

What are the basic principles of therapy? 08/06/2020

Recombinant protein therapy should follow the basic principles of individuality, pertinence, safety and effectiveness.

Have the pharmacokinetics and pharmacodynamics of s been clearly understood? 06/09/2020

The pharmacokinetics and pharmacodynamics of s have been clearly understood, but they still need to be explored and perfected in clinical practice.

Is the production cost of high? 03/15/2020

The production cost of is relatively high, but because of its therapeutic effect and broad application prospects, it has become an important field in the pharmaceutical industry.

Does the application of s require individual adjustment? 02/23/2020

The application of s needs to be adjusted individually according to the specific situation of the patient in order to achieve the optimal therapeutic effect.

Can s be used in the treatment of neurological diseases? 02/06/2020

Recombinant proteins have been used in the treatment of various neurological diseases, such as multiple sclerosis and Parkinson's disease.

What is the application prospect of ? 09/27/2019

As a new drug therapy technology, has broad application prospects, and more s may be used in the future.

Is the easily broken down in the body? 04/13/2019

Recombinant proteins are subject to various metabolic and decomposition influences in the body, and different proteins will have different stability and half-lives.

Are clinical trials of s strictly regulated? 02/09/2019

Clinical trials of s are subject to strict compliance with relevant ethical and regulatory requirements and are subject to regulatory approval and supervision.

Customer Reviews (4)

Write a review
Reviews
05/22/2022

    Short half-life and high clearance.

    09/17/2021

      APCS produced by them has low preparation cost and high cost performance.

      08/17/2021

        Stability of APCS is very good, and it can maintain stable performance even in extreme environments, which shows its excellent adaptability and reliability.

        08/03/2019

          Production process is very much in line with the principle of green production.

          Ask a Question for All APCS Products

          Required fields are marked with *

          My Review for All APCS Products

          Required fields are marked with *

          0

          Inquiry Basket

          cartIcon
          logo

          FOLLOW US

          Terms and Conditions        Privacy Policy

          Copyright © 2024 Creative BioMart. All Rights Reserved.

          Contact Us

          • /

          Stay Updated on the Latest Bioscience Trends