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Recombinant Rat Csf2

Cat. No.: Csf2-94R
Product Overview: Recombinant Rat Granulocyte Macrophage Colony Stimulating Factor produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14590.65 Dalton. Recombinant Rat GM-CSF is purified by proprietary chromatographic techniques.
Description: GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Source: Escherichia Coli.
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation: Rat GM-CSF was lyophilized with no additives.
Solubility: It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Specific Activity: The ED50 range = 0.001-0.01 ng/ml, determined by the dose dependent proliferation of murine MC/9 cells.
Protein Content: GMCSF quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.966 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard.
Stability: Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GMCSF should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name: Csf2 colony stimulating factor 2 (granulocyte-macrophage) [ Rattus norvegicus ]
Synonyms: Csf2; colony stimulating factor 2 (granulocyte-macrophage); Gmcsf; Gm-csf; colony stimulating factor 2
Gene ID: 116630
mRNA Refseq: XM_340799
Protein Refseq: XP_340800
UniProt ID: P48750
Chromosome Location: 10q22
Pathway: Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Hematopoietic cell lineage; Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity; T cell receptor signaling pathway
Function: cytokine activity; granulocyte macrophage colony-stimulating factor receptor binding; growth factor activity; protein binding

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