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Recombinant Human WRAP53 293 Cell Lysate

Cat.No.: WRAP53-283HCL
Description: Antigen standard for WD repeat containing, antisense to TP53 (WRAP53), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: WRAP53 WD repeat containing, antisense to TP53 [ Homo sapiens ]
Official Symbol: WRAP53
Synonyms: WRAP53; WD repeat containing, antisense to TP53; WD repeat domain 79 , WDR79; telomerase Cajal body protein 1; FLJ10385; TCAB1; telomerase cajal body protein 1; WD encoding RNA antisense to p53; WD repeat domain 79; WD40 protein Wrap53; WD repeat-containing protein 79; WD40 repeat-containing protein encoding RNA antisense to p53; DKCB3; WDR79;
Gene ID: 55135
mRNA Refseq: NM_001143990
Protein Refseq: NP_001137462
MIM: 612661
UniProt ID: Q9BUR4
Chromosome Location: 17p13.1
Pathway: Cell Cycle, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; Extension of Telomeres, organism-specific biosystem; Telomere Extension By Telomerase, organism-specific biosystem; Telomere Maintenance, organism-specific biosystem;

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