"TNNT2" Related Products

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Recombinant Human TNNT2 293 Cell Lysate

Cat.No.: TNNT2-879HCL
Description: Antigen standard for troponin T type 2 (cardiac) (TNNT2), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: TNNT2 troponin T type 2 (cardiac) [ Homo sapiens ]
Official Symbol: TNNT2
Synonyms: TNNT2; troponin T type 2 (cardiac); cardiomyopathy, hypertrophic 2 , CMH2, troponin T2, cardiac; troponin T, cardiac muscle; troponin T2, cardiac; cardiac muscle troponin T; cardiomyopathy, hypertrophic 2; cardiomyopathy, dilated 1D (autosomal dominant); CMH2; RCM3; TnTC; cTnT; CMPD2; LVNC6; MGC3889;
Gene ID: 7139
mRNA Refseq: NM_001001431
Protein Refseq: NP_001001431
MIM: 191045
UniProt ID: P45379
Chromosome Location: 1q32
Pathway: Cardiac muscle contraction, organism-specific biosystem; Cardiac muscle contraction, conserved biosystem; Dilated cardiomyopathy, organism-specific biosystem; Dilated cardiomyopathy, conserved biosystem; Hypertrophic cardiomyopathy (HCM), organism-specific biosystem; Hypertrophic cardiomyopathy (HCM), conserved biosystem; Muscle contraction, organism-specific biosystem;

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