"SCCPDH" Related Products


Recombinant Human SCCPDH 293 Cell Lysate

Cat.No.: SCCPDH-2045HCL
Description: Antigen standard for saccharopine dehydrogenase (putative) (SCCPDH) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SCCPDH saccharopine dehydrogenase (putative) [ Homo sapiens ]
Official Symbol: SCCPDH
Synonyms: SCCPDH; saccharopine dehydrogenase (putative); saccharopine dehydrogenase-like oxidoreductase; CGI 49; NET11; probable saccharopine dehydrogenase; CGI-49; FLJ43187; RP11-439E19.2;
Gene ID: 51097
mRNA Refseq: NM_016002
Protein Refseq: NP_057086
UniProt ID: Q8NBX0
Chromosome Location: 1q44
Function: nucleotide binding; oxidoreductase activity;
Products Types ◆ Recombinant Protein

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