"RPL6" Related Products

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Recombinant Human RPL6 293 Cell Lysate

Cat.No.: RPL6-2191HCL
Description: Antigen standard for ribosomal protein L6 (RPL6), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RPL6 ribosomal protein L6 [ Homo sapiens ]
Official Symbol: RPL6
Synonyms: RPL6; ribosomal protein L6; TXREB1; 60S ribosomal protein L6; L6; TAXREB107; DNA-binding protein TAXREB107; neoplasm-related protein C140; tax-responsive enhancer element-binding protein 107; SHUJUN-2;
Gene ID: 6128
mRNA Refseq: NM_000970
Protein Refseq: NP_000961
MIM: 603703
UniProt ID: Q02878
Chromosome Location: 12q24.13
Pathway: Cap-dependent Translation Initiation, organism-specific biosystem; Cytoplasmic Ribosomal Proteins, organism-specific biosystem; Disease, organism-specific biosystem; Eukaryotic Translation Elongation, organism-specific biosystem; Eukaryotic Translation Initiation, organism-specific biosystem; Eukaryotic Translation Termination, organism-specific biosystem; Formation of a pool of free 40S subunits, organism-specific biosystem;
Function: DNA binding; RNA binding; structural constituent of ribosome;

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