"RHOXF1" Related Products

Recombinant Human RHOXF1 293 Cell Lysate

Cat.No.: RHOXF1-2346HCL
Description: Antigen standard for Rhox homeobox family, member 1 (RHOXF1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RHOXF1 Rhox homeobox family, member 1 [ Homo sapiens ]
Official Symbol: RHOXF1
Synonyms: RHOXF1; Rhox homeobox family, member 1; rhox homeobox family member 1; OTEX; PEPP1; PEPP subfamily gene 1; paired-like homeobox protein OTEX; paired-like homeobox protein PEPP-1; ovary-, testis- and epididymis-expressed gene protein; MGC119030; MGC119033;
Gene ID: 158800
mRNA Refseq: NM_139282
Protein Refseq: NP_644811
MIM: 300446
UniProt ID: Q8NHV9
Chromosome Location: Xq24
Function: DNA binding; sequence-specific DNA binding transcription factor activity; transcription regulatory region sequence-specific DNA binding;

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