"RGS9" Related Products

Recombinant Human RGS9 293 Cell Lysate

Cat.No.: RGS9-2367HCL
Description: Antigen standard for regulator of G-protein signaling 9 (RGS9), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RGS9 regulator of G-protein signaling 9 [ Homo sapiens ]
Official Symbol: RGS9
Synonyms: RGS9; regulator of G-protein signaling 9; regulator of G protein signalling 9; MGC26458; MGC111763; PERRS; regulator of G protein signalling 9L; regulator of G protein signaling 9L; RGS9L; regulator of G-protein signalling 9;
Gene ID: 8787
mRNA Refseq: NM_001081955
Protein Refseq: NP_001075424
MIM: 604067
UniProt ID: O75916
Chromosome Location: 17q24
Pathway: Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Cocaine addiction, organism-specific biosystem; Cocaine addiction, conserved biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem; Phototransduction, organism-specific biosystem;
Function: GTPase activator activity; protein complex binding; signal transducer activity;

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