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Recombinant Human RBCK1 293 Cell Lysate

Cat.No.: RBCK1-2487HCL
Description: Antigen standard for RanBP-type and C3HC4-type zinc finger containing 1 (RBCK1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RBCK1 RanBP-type and C3HC4-type zinc finger containing 1 [ Homo sapiens ]
Official Symbol: RBCK1
Synonyms: RBCK1; RanBP-type and C3HC4-type zinc finger containing 1; C20orf18, chromosome 20 open reading frame 18; ranBP-type and C3HC4-type zinc finger-containing protein 1; RBCK2; RNF54; UBCE7IP3; XAP4; ZRANB4; RING finger protein 54; HBV associated factor 4; HBV-associated factor 4; RBCC protein interacting with PKC1; heme-oxidized IRP2 ubiquitin ligase 1; hepatitis B virus X-associated protein 4; ubiquitin conjugating enzyme 7 interacting protein 3; ubiquitin-conjugating enzyme 7-interacting protein 3; XAP3; HOIL1; HOIL-1; C20orf18;
Gene ID: 10616
mRNA Refseq: NM_006462
Protein Refseq: NP_006453
MIM: 610924
UniProt ID: Q9BYM8
Chromosome Location: 20p13
Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem;
Function: DNA binding; ligase activity; metal ion binding; protein binding; protein kinase C binding; NOT sequence-specific DNA binding transcription factor activity; ubiquitin binding; ubiquitin-protein ligase activity; zinc ion binding;

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