"RASL10A" Related Products

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Recombinant Human RASL10A 293 Cell Lysate

Cat.No.: RASL10A-2503HCL
Description: Antigen standard for RAS-like, family 10, member A (RASL10A), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RASL10A RAS-like, family 10, member A [ Homo sapiens ]
Official Symbol: RASL10A
Synonyms: RASL10A; RAS-like, family 10, member A; ras-like protein family member 10A; RRP22; ras-like protein RRP22; RAS-related on chromosome 22; ras-related protein on chromosome 22;
Gene ID: 10633
mRNA Refseq: NM_006477
Protein Refseq: NP_006468
MIM: 602220
UniProt ID: Q92737
Chromosome Location: 22q12.2
Function: GTP binding; GTPase activity; nucleotide binding;

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