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Recombinant Human RARB 293 Cell Lysate

Cat.No. : RARB-2514HCL
  • Specification
  • Gene Information
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Description : Antigen standard for retinoic acid receptor, beta (RARB), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : RARB retinoic acid receptor, beta [ Homo sapiens ]
Official Symbol : RARB
Synonyms : RARB; retinoic acid receptor, beta; retinoic acid receptor beta; HAP; NR1B2; RRB2; RAR-beta; RAR-epsilon; HBV-activated protein; retinoic acid receptor beta 2; retinoic acid receptor beta 4; retinoic acid receptor beta 5; hepatitis B virus activated protein; retinoic acid receptor beta variant 1; retinoic acid receptor beta variant 2; retinoic acid receptor, beta polypeptide; nuclear receptor subfamily 1 group B member 2; MGC97363;
Gene ID : 5915
mRNA Refseq : NM_000965
Protein Refseq : NP_000956
MIM : 180220
UniProt ID : P10826
Chromosome Location : 3p24
Pathway : Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Non-small cell lung cancer, organism-specific biosystem; Non-small cell lung cancer, conserved biosystem; Nuclear Receptor transcription pathway, organism-specific biosystem; Nuclear Receptors, organism-specific biosystem; Nuclear receptors in lipid metabolism and toxicity, organism-specific biosystem;
Function : DNA binding; metal ion binding; receptor activity; retinoic acid receptor activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; steroid hormone receptor activity; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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