"RALB" Related Products

Recombinant Human RALB 293 Cell Lysate

Cat.No.: RALB-2543HCL
Description: Antigen standard for v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein) (RALB) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RALB v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein) [ Homo sapiens ]
Official Symbol: RALB
Synonyms: RALB; v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein); ras-related protein Ral-B; RAS-like protein B; GTP binding protein;
Gene ID: 5899
mRNA Refseq: NM_002881
Protein Refseq: NP_002872
MIM: 179551
UniProt ID: P11234
Chromosome Location: 2q14.2
Pathway: CXCR4-mediated signaling events, organism-specific biosystem; EGFR1 Signaling Pathway, organism-specific biosystem; NGF signalling via TRKA from the plasma membrane, organism-specific biosystem; Pancreatic cancer, organism-specific biosystem; Pancreatic cancer, conserved biosystem; Pathways in cancer, organism-specific biosystem; Signal Transduction, organism-specific biosystem;
Function: GTP binding; GTP binding; GTPase activity; GTPase activity; nucleotide binding; protein binding;

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