"RAD51" Related Products


Recombinant Human RAD51 293 Cell Lysate

Cat.No.: RAD51-2556HCL
Description: Antigen standard for RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RAD51 RAD51 homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol: RAD51
Synonyms: RAD51; RAD51 homolog (S. cerevisiae); RAD51 (S. cerevisiae) homolog (E coli RecA homolog) , RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) , RAD51A, RECA; DNA repair protein RAD51 homolog 1; BRCA1/BRCA2 containing complex; subunit 5; BRCC5; HsRad51; HsT16930; RAD51 homolog A; RecA-like protein; recombination protein A; RecA, E. coli, homolog of; RAD51 homolog (RecA homolog, E. coli); BRCA1/BRCA2-containing complex, subunit 5; RECA; MRMV2; HRAD51; RAD51A;
Gene ID: 5888
mRNA Refseq: NM_001164269
Protein Refseq: NP_001157741
MIM: 179617
UniProt ID: Q06609
Chromosome Location: 15q15.1
Pathway: Assembly of the RAD51-ssDNA nucleoprotein complex, organism-specific biosystem; BARD1 signaling events, organism-specific biosystem; DNA Repair, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem; Homologous DNA pairing and strand exchange, organism-specific biosystem;
Function: ATP binding; ATP binding; DNA-dependent ATPase activity; damaged DNA binding; double-stranded DNA binding; double-stranded DNA binding; identical protein binding; nucleoside-triphosphatase activity; nucleotide binding; protein C-terminus binding; protein binding; single-stranded DNA binding; single-stranded DNA-dependent ATPase activity;

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