Recombinant Human PPP3CC 293 Cell Lysate

• Cat.No.: PPP3CC-2913HCL

Specification

• Description: Antigen standard for protein phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform (PPP3CC) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PPP3CC protein phosphatase 3, catalytic subunit, gamma isozyme [ Homo sapiens ]
• Official Symbol: PPP3CC
• Synonyms: PPP3CC; protein phosphatase 3, catalytic subunit, gamma isozyme; protein phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform , protein phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform (calcineurin A gamma); serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform; calcineurin A gamma; CALNA3; PP2Bgamma; protein phosphatase 2B; catalytic subunit; gamma isoform; CAM-PRP catalytic subunit; calcineurin, testis-specific catalytic subunit; calmodulin-dependent calcineurin A subunit gamma isoform; protein phosphatase 2B, catalytic subunit, gamma isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform; CNA3
• Gene ID: 5533
• mRNA Refseq: NM_001243974
• Protein Refseq: NP_001230903
• MIM: 114107
• UniProt ID: P48454
• Chromosome Location: 8p21.3
• Pathway: Activation of BAD and translocation to mitochondria, organism-specific biosystem; Activation of BH3-only proteins, organism-specific biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Amphetamine addiction, organism-specific biosystem; Amphetamine addiction, conserved biosystem; Amyotrophic lateral sclerosis (ALS), organism-specific biosystem
• Function: calmodulin binding; hydrolase activity; metal ion binding; phosphoprotein phosphatase activity