"PELI2" Related Products

Recombinant Human PELI2 293 Cell Lysate

Cat.No.: PELI2-3303HCL
Description: Antigen standard for pellino homolog 2 (Drosophila) (PELI2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PELI2 pellino E3 ubiquitin protein ligase family member 2 [ Homo sapiens ]
Official Symbol: PELI2
Synonyms: PELI2; pellino E3 ubiquitin protein ligase family member 2; pellino (Drosophila) homolog 2 , pellino homolog 2 (Drosophila); E3 ubiquitin-protein ligase pellino homolog 2; pellino-2; pellino homolog 2; protein pellino homolog 2;
Gene ID: 57161
mRNA Refseq: NM_021255
Protein Refseq: NP_067078
UniProt ID: Q9HAT8
Chromosome Location: 14q21
Pathway: Activated TLR4 signalling, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; IRAK1 recruits IKK complex, organism-specific biosystem; IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Interleukin-1 signaling, organism-specific biosystem;
Function: ligase activity; protein binding;
Products Types ◆ Recombinant Protein

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